Mouse Reg3B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5110
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Reg3B
Glu27-Gly175
Accession # P35230
Glu27-Gly175
Accession # P35230
Specificity
Detects mouse Reg3B in direct ELISAs and Western blots. In direct ELISAs, approximately 75% cross-reactivity with recombinant rat (rr) Reg3B is observed. In Western blots approximately 30% cross-reactivity with rrReg3B is observed. In direct ELISAs and Western blots, less than 1% cross‑reactivity with rrReg3A, recombinant mouse (rm) Reg3A, and rmReg3G is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Reg3B Antibody
Reg3B in Mouse Small Intestine.
Reg3B was detected in immersion fixed frozen sections of mouse small intestine using 10 µg/mL Sheep Anti-Mouse Reg3B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5110) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse Reg3B Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse small intestine
Sample: Immersion fixed frozen sections of mouse small intestine
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Reg3B
Sample: Recombinant Mouse Reg3B
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF5110 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg3B
Long Name
Regenerating Islet-derived IIIb
Alternate Names
PAP1
Gene Symbol
REG3B
UniProt
Additional Reg3B Products
Product Documents for Mouse Reg3B Antibody
Product Specific Notices for Mouse Reg3B Antibody
For research use only
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