Mouse RGM-B Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF3597

R&D Systems, part of Bio-Techne
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BAF3597
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AF3597
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, Western Blot

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all RGM-B Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RGM-B
Gly49-Ser414
Accession # Q7TQ33

Specificity

Detects mouse RGM‑B in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse (rm) RGM-C is observed and less than 2% cross‑reactivity with rmRGM‑A is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse RGM-B Biotinylated Antibody

RGM-B antibody in Neuro-2A Mouse Cell Line by Immunocytochemistry (ICC).

RGM‑B in Neuro‑2A Mouse Cell Line.

RGM-B was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Sheep Anti-Mouse RGM-B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3597) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse RGM-B Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Neuro-2A mouse neuroblastoma cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Neuro2A mouse neuroblastoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse RGM-B (Catalog # 3597-RG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-B

RGM-B, also known as DRAGON, is a 40 kDa member of the repulsive guidance molecule (RGM) family of GPI-linked neuronal and muscle membrane proteins (1, 2). It is synthesized as a preproprotein that consists of a 48 amino acid (aa) signal sequence, a 367 aa mature region, and a 21 aa C-terminal prosegment (3). RGM-B contains two potential N-linked glycosylation sites and an abbreviated von Willebrand factor domain. Potential proteolytic cleavage within the VWF domain is supported by R&D Systems’ in house data (4). Within the region following the VWF domain, mouse RGM-B shares 49% and 43% aa sequence identity with RGM-A and RGM-C, respectively. It shares 90%, 79%, 92%, and 93% aa sequence identity with bovine, chicken, human, and rhesus macaque RGM-B, respectively. RGM-B is expressed in the developing and adult nervous system, particularly in the dorsal root ganglia and mantle layer of the spinal cord (3-5). In mouse, it shows a complementary, non-overlapping distribution with RGM-A (2-5). RGM-B is also expressed in fetal and adult enteric ganglia and in postnatal intestinal epithelium (6). RGM-B expression has been detected in neuronal cell bodies and proximal axonal segments (4) but is also present on the cell surface, where it interacts homophilically and mediates neuronal adhesion (3). RGM-B additionally functions as a BMP coreceptor. It directly binds BMP-2 and -4 but not other TGF-beta family proteins (7). RGM-B associates with BMP type I (ALK-2, -3, -6) and type II (Activin RIIA, Activin RIIB) receptors and enhances BMP signaling (7).

References

  1. Monnier, P.P. et al. (2002) Nature 419:392.
  2. Schmidtmer, J. and D. Engelkamp (2004) Gene Exp. Patterns 4:105.
  3. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  4. Niederkofler, V. et al. (2004) J. Neurosci. 24:808.
  5. Oldekamp, J. et al. (2004) Gene Exp. Patterns 4:283.
  6. Metzger, M. et al. (2005) Dev. Dyn. 234:169.
  7. Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.

Long Name

Repulsive Guidance Molecule B

Alternate Names

Dragon, RGMB

Entrez Gene IDs

285704 (Human); 68799 (Mouse)

Gene Symbol

RGMB

UniProt

Q7TQ33

Additional RGM-B Products

Product Documents for Mouse RGM-B Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mouse RGM-B Biotinylated Antibody

For research use only

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