Mouse S100A9 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2065
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Simple Western, Western Blot
Cited:
Confocal Microscopy, ELISA Development, Functional Assay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse S100A9
Ala2-Lys113
Accession # P31725
Ala2-Lys113
Accession # P31725
Specificity
Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant mouse S100A10 and recombinant human S100B is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse S100A9 Antibody
Detection of Mouse S100A9 by Western Blot.
Western blot shows lysate of mouse lung tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A9 in XB2 Mouse Cell Line.
S100A9 was detected in immersion fixed XB2 mouse teratoma keratinocyte cell line using 10 µg/mL Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counte-rstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
S100A9 in Mouse Splenocytes.
S100A9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2065) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse S100A9 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed XB2 mouse teratoma keratinocyte cell line and mouse splenocytes
Sample: Immersion fixed XB2 mouse teratoma keratinocyte cell line and mouse splenocytes
Immunohistochemistry
0.3-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen
Sample: Perfusion fixed frozen sections of mouse spleen
Simple Western
2 µg/mL
Sample: Mouse lung tissue and mouse spleen tissue
Sample: Mouse lung tissue and mouse spleen tissue
Western Blot
0.2 µg/mL
Sample: Mouse lung tissue
Sample: Mouse lung tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.6 using AF2065 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A9
Long Name
S100 Calcium Binding Protein A9
Alternate Names
Calgranulin B, MRP-14
Gene Symbol
S100A9
UniProt
Additional S100A9 Products
Product Documents for Mouse S100A9 Antibody
Product Specific Notices for Mouse S100A9 Antibody
For research use only
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