Mouse S1P5/EDG-8 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9084P

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1196A

View all S1P5/EDG-8 Antibodies »

Product Specifications

Immunogen

Mouse S1P5/EDG-8 peptide corresponding to the N-terminal extracellular sequence
Accession # Q91X56

Specificity

Detects mouse S1P5/EDG-8 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse S1P5/EDG-8 PE-conjugated Antibody

Detection of S1P5/EDG-8 antibody in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP antibody by Flow Cytometry.

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP were stained with either (A) Rabbit Anti-Mouse S1P5/EDG‑8 PE‑conjugated Monoclonal Antibody (Catalog # FAB9084P) or (B) Normal Rabbit IgG Phycoerythrin Control (Catalog # IC1051P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse S1P5/EDG-8 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P5/EDG-8 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P5/EDG-8

S1P5 (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P1 -S1P4 (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P5 is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P5 is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C+ (in human CD14+CD16-) monocytes, pre- and mature oligodendrocytes, and CD27-CD116+ (mature) NK cells. S1P5 appears to play a role in cell trafficking. On monocytes and NK cells, S1P5 promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P5 also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P5 appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P5 shares 96% and 94% aa sequence identity with rat and human S1P5 extracellular domains, respectively.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q91X56

Additional S1P5/EDG-8 Products

Product Documents for Mouse S1P5/EDG-8 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse S1P5/EDG-8 PE-conjugated Antibody

For research use only

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