Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mouse S1P₅. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse S1P₅/EDG-8 Monoclonal Antibody (Catalog # MAB9084) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for S1P₅/EDG-8 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of S1P₅/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P₅/EDG-8 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse S1P₅/EDG-8 and eGFP were stained with and either (A) Rabbit Anti-Mouse S1P₅/EDG-8 Monoclonal Antibody (Catalog # MAB9084) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse S1P₅/EDG-8 Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P₅/EDG-8

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P₅/EDG-8

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P5/EDG-8

S1P₅ (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P₁-S1P₄ (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P₅ is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P₅ is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C⁺ (in human CD14⁺CD16^-) monocytes, pre- and mature oligodendrocytes, and CD27^-CD116⁺ (mature) NK cells. S1P₅ appears to play a role in cell trafficking. On monocytes and NK cells, S1P₅ promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P₅ also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P₅ appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P₅ shares 96% and 94% aa sequence identity with rat and human S1P₅ extracellular domains, respectively.

Long Name

Endothelial Differentiation Gene 8

Alternate Names

EDG8, LPB4, S1PR5, SPPR-1, SPPR-2

Entrez Gene IDs

53637 (Human); 94226 (Mouse); 60399 (Rat)

Gene Symbol

S1PR5

UniProt

Q91X56

Additional S1P5/EDG-8 Products

Product Documents for Mouse S1P₅/EDG-8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse S1P₅/EDG-8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse S1P5/EDG-8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9084

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all S1P5/EDG-8 Antibodies »

Product Summary for Mouse S1P5/EDG-8 Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse S1P5/EDG-8 Antibody

Detection of Mouse S1P5/EDG‑8 by Western Blot.

Detection of S1P5/EDG‑8 in HEK293 Human Cell Line Transfected with Mouse S1P5/EDG-8 and eGFP by Flow Cytometry.

Applications for Mouse S1P5/EDG-8 Antibody