Mouse Semaphorin 4B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6239
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4B
Leu31-Glu703
Accession # Q62179
Leu31-Glu703
Accession # Q62179
Specificity
Detects mouse Semaphorin 4B in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human Semaphorin 4B is observed and less than 2% cross‑reactivity with recombinant mouse (rm) Semaphorin 4A and rmSemaphorin 4C is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse Semaphorin 4B Antibody
Detection of Mouse Semaphorin 4B by Western Blot.
Western blot shows lysates of mouse pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Mouse Semaphorin 4B Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse pancreas tissue
Sample: Mouse pancreas tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4B
Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular region (aa 31‑703) that is characterized by one Sema domain (aa 57‑492), a PSI region (aa 512‑582), and an Ig‑like C2‑type domain (aa 589‑649). There are three phosphoserines in the cytoplasmic tail. Over aa 31‑703, mouse Sema4B shares 86% and 95% aa identity with human and rat Sema4B, respectively.
Alternate Names
SEMA4B, SEMAC, SemC
Gene Symbol
SEMA4B
UniProt
Additional Semaphorin 4B Products
Product Documents for Mouse Semaphorin 4B Antibody
Product Specific Notices for Mouse Semaphorin 4B Antibody
For research use only
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