Mouse Semaphorin 6A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1615
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey), Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464
Gly19-Thr649
Accession # O35464
Specificity
Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Semaphorin 6A is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3F, and rmSemaphorin 7A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Semaphorin 6A Antibody
Detection of Semaphorin 6A in Mouse Intestine.
Semaphorin 6A was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Semaphorin 6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1615) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse Semaphorin 6A Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Mouse Intestine
Sample: Immersion fixed paraffin-embedded sections of Mouse Intestine
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Semaphorin 6A
Sample: Recombinant Mouse Semaphorin 6A
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 6A
Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal (1).
References
- Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.
Alternate Names
Sema6A
Gene Symbol
SEMA6A
UniProt
Additional Semaphorin 6A Products
Product Documents for Mouse Semaphorin 6A Antibody
Product Specific Notices for Mouse Semaphorin 6A Antibody
For research use only
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