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Mouse Serpin E2/PN1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2175

R&D Systems, part of Bio-Techne
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BAF2175

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E2/PN1
Ser20-Pro397
Accession # Q07235

Specificity

Detects mouse Serpin E2/PN1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fas, recombinant mouse Fas, and recombinant feline Fas (under non-reducing conditions) is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Serpin E2/PN1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E2/PN1

Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over-expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over-expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
  3. Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
  4. Meins, M. et al. (2001) J. Neurosci. 21:8830.
  5. Buchholz, M. et al. (2003) Cancer Res. 63:4945.
  6. Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.

Alternate Names

GDN, GdNPF, Proteinase Nexin 1

Entrez Gene IDs

5270 (Human); 20720 (Mouse)

Gene Symbol

SERPINE2

UniProt

Additional Serpin E2/PN1 Products

Product Documents for Mouse Serpin E2/PN1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Serpin E2/PN1 Biotinylated Antibody

For research use only

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