Mouse Serpin E2/PN1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2175
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Neutralization, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E2/PN1
Ser20-Pro397
Accession # Q07235
Ser20-Pro397
Accession # Q07235
Specificity
Detects mouse Serpin E2/PN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin I2, recombinant mouse (rm) Serpin C1, rmSerpin D1, and rmSerpin F2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Mouse Serpin E2/PN1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI)
Sample: Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI)
Neutralization
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin E2/PN1
Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
- Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
- Meins, M. et al. (2001) J. Neurosci. 21:8830.
- Buchholz, M. et al. (2003) Cancer Res. 63:4945.
- Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.
Alternate Names
GDN, GdNPF, Proteinase Nexin 1
Gene Symbol
SERPINE2
UniProt
Additional Serpin E2/PN1 Products
Product Documents for Mouse Serpin E2/PN1 Antibody
Product Specific Notices for Mouse Serpin E2/PN1 Antibody
For research use only
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