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Mouse SOD3/EC-SOD Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4817

R&D Systems, part of Bio-Techne
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AF4817
AF4817-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

E. coli-derived recombinant mouse SOD3/EC‑SOD
Ser25-Thr251
Accession # O09164

Specificity

Detects mouse SOD3/EC‑SOD in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse SOD1 or SOD2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse SOD3/EC-SOD Antibody

Detection of Mouse SOD3/EC-SOD antibody by Western Blot.

Detection of Mouse SOD3/EC-SOD by Western Blot.

Western blot shows lysates of mouse lung and kidney tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Mouse SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant mouse SOD1, SOD2, and SOD3 (5 ng/lane) were included. A specific band for SOD3/EC-SOD was detected at approxi-mately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Mouse SOD3/EC-SOD by Simple WesternTM.

Simple Western shows lysates of mouse aorta, loaded at 0.5 mg/ml. A specific band was detected for SOD3/EC-SOD at approximately 40 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Applications for Mouse SOD3/EC-SOD Antibody

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: Mouse aorta tissue

Western Blot

1 µg/mL
Sample: Mouse lung and kidney tissue

Reviewed Applications

Read 1 review rated 5 using AF4817 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD3/EC-SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Mouse SOD3 shares ~66% and 82% sequence homology with human and rat SOD3, respectively. SOD3 is a secretory protein and is synthesized with a putative 24-amino acid signal peptide preceding the 227 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.

Long Name

Superoxide Dismutase-3

Alternate Names

EC-SOD, ECSOD

Entrez Gene IDs

6649 (Human); 20657 (Mouse); 25352 (Rat)

Gene Symbol

SOD3

UniProt

Additional SOD3/EC-SOD Products

Product Documents for Mouse SOD3/EC-SOD Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse SOD3/EC-SOD Antibody

For research use only

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