Mouse ST2/IL-33R Alexa Fluor® 700-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB10041N
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # 245707
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
Ser27-Arg332
Accession # P14719
Ser27-Arg332
Accession # P14719
Specificity
Detects mouse ST2/IL-33 R in ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse ST2/IL-33R Alexa Fluor® 700-conjugated Antibody
Detection of ST2/IL-33 R in P815 Mouse Cell Line by Flow Cytometry.
P815 mouse mastocytoma cell line was stained with Rat Anti-Mouse ST2/IL-33 R Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB10041N, filled histogram) or isotype control antibody (IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of ST2/IL-33 R in Raw264.7 Mouse Cell Line by Flow Cytometry.
Raw264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse ST2/IL-33 R Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB10041N, filled histogram) or isotype control antibody (IC013N, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.Applications for Mouse ST2/IL-33R Alexa Fluor® 700-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
5 µL/106 cells
Sample: P815 mouse mastocytoma cell line and Raw264.7 mouse monocyte/macrophage cell line
Sample: P815 mouse mastocytoma cell line and Raw264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ST2/IL-33R
ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2 (9, 12‑14).
References
- Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
- Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
- Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
- Schmitz, J. et al. (2005) Immunity 23:479.
- Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
- Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
- Weinberg, E.O. et al. (2002) Circulation 106:2961.
- Sanada S. et al. (2007) J. Clin. Invest. 117:1538
- Palmer, G. et al. (2008) Cytokine 42:358.
- Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
- Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
- Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
- Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
- Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name
Interleukin 33 Receptor
Alternate Names
DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1
Gene Symbol
IL1RL1
UniProt
Additional ST2/IL-33R Products
Product Specific Notices for Mouse ST2/IL-33R Alexa Fluor® 700-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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