Mouse Stella/Dppa3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2566
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Stella/Dppa3
Met1-Asn150
Accession # Q8QZY3
Met1-Asn150
Accession # Q8QZY3
Specificity
Detects mouse Stella/Dppa3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Stella is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Stella/Dppa3 Antibody
Stella/Dppa3 in mouse ovary tissue.
Stella/Dppa3 was detected in perfusion fixed frozen sections of mouse ovary tissue using Goat Anti-Mouse Stella/Dppa3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2566) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to oocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse Stella/Dppa3 Antibody
Application
Recommended Usage
Immunohistochemistry
1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary
Sample: Perfusion fixed frozen sections of mouse ovary
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Stella/Dppa3
Sample: Recombinant Mouse Stella/Dppa3
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF2566 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Stella/Dppa3
Long Name
Developmental Pluripotency-associated Protein 3
Alternate Names
Dppa3
Gene Symbol
DPPA3
UniProt
Additional Stella/Dppa3 Products
Product Documents for Mouse Stella/Dppa3 Antibody
Product Specific Notices for Mouse Stella/Dppa3 Antibody
For research use only
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