Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3454

R&D Systems, part of Bio-Techne
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AF3454
AF3454-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Sulfatase Modifying Factor 2/SUMF2 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Sulfatase Modifying Factor 2/SUMF2
Gln34-Leu308
Accession # Q8BPG6

Specificity

Detects mouse Sulfatase Modifying Factor 2/SUMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) SUMF2 and less than 5% cross-reactivity with rhSUMF1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Sulfatase Modifying Factor 2/SUMF2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 2/SUMF2

Mouse SUMF2 is a 30 kDa member of the sulfatase-modifying factor family. It is synthesized as a 275 amino acid (aa) glycoprotein that is localized to the ER. It contains multiple calcium-binding sites and three (sub)domains of varying length and ill-defined structure (aa 38‑298). There is one potential alternate start site at aa 118 that would generate a 190 aa precursor. The founding family member, SUMF1, posttranslationally activates newly synthesized sulfatases by converting a Cysteine to formylGly in the catalytic site. SUMF2 apparently has no such activity. It forms homodimers and heterodimers with SUMF1. As a heterodimer, it downmodulates SUMF1 activity. Mature mouse SUMF2 is 85% and 93% aa identical to human and rat SUMF2, respectively.

Alternate Names

FGE2

Entrez Gene IDs

25870 (Human)

Gene Symbol

SUMF2

UniProt

Q8BPG6

Additional Sulfatase Modifying Factor 2/SUMF2 Products

Product Documents for Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Sulfatase Modifying Factor 2/SUMF2 Antibody

For research use only

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