Mouse Syndecan-1/CD138 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2966A

R&D Systems, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 300506

View all Syndecan-1/CD138 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Syndecan‑1/CD138 isoform 1
Gln18-Glu252
Accession # P18828

Specificity

Detects mouse Syndecan‑1/CD138 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Syndecan-1, recombinant mouse (rm) Syndecan-3 or rmSyndecan-4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse Syndecan-1/CD138 APC-conjugated Antibody

Detection of Syndecan-1/CD138 antibody in T1165 Mouse Cell Line antibody by Flow Cytometry.

Detection of Syndecan-1/CD138 in T1165 Mouse Cell Line by Flow Cytometry.

T1165 mouse plasmacytoma cell line treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) was stained with Rat Anti-Mouse Syndecan-1/CD138 APC-conjugated Monoclonal Antibody (Catalog # FAB2966A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Syndecan-1/CD138 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: T1165 mouse plasmacytoma cell line treated with Recombinant Human IL‑6 (Catalog # 206-IL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Syndecan-1/CD138

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the Syndecan family of Type 1 transmembrane proteins (1, 2). The four Syndecan family members are major carriers of Heparan Sulfate (HS) and Chondroitin Sulfate Glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 22 aa signal sequence, a 233 aa extracellular domain (ECD) that includes three closely spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 21 aa TM segment, and a 35 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site (4). The ECD is variably modified by GAGs, producing molecular weights of 120-200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Mouse Syndecan-1 ECD shares 70% and 87% aa identity with the ECD of human and rat Syndecan-1, respectively. Alternative splicing in mouse generates an isoform with an internal deletion of 44 aa from the ECD (5). Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (6, 7). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (6-10). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (7, 9, 11). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (12-14). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (14, 15). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (6).

References

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
  3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  4. Saunders, S. et al. (1989) J. Cell Biol. 108:1547.
  5. Romaris, M. et al. (1999) J. Biol. Chem. 274:18667.
  6. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
  7. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
  8. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
  9. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
  10. Vanhoutte, D. et al. (2007) Circulation 115:475.
  11. Li, Q. et al. (2002) Cell 111:635.
  12. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
  13. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
  14. Derksen, P.W.B. et al. (2002) Blood 99:1405.
  15. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Alternate Names

CD138, SDC1, Syndecan1

Entrez Gene IDs

6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

Gene Symbol

SDC1

UniProt

P18828

Additional Syndecan-1/CD138 Products

Product Documents for Mouse Syndecan-1/CD138 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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