Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR gamma/d Monoclonal Antibody (Catalog # MAB7297) or (B) Normal Hamster IgG Control Antibody followed by Phycoerythrin-conjugated Anti-Hamster IgG Secondary Antibody.

Applications for Mouse TCR gamma/delta Antibody

Application

Recommended Usage

CyTOF-reported

Spitzer, M. et al. (2015) Science 349: 1259425.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TCR gamma/delta

The gamma delta T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3 delta glycoprotein subunits of the Ig superfamily. T gamma delta cells develop as a minor population in the thymus and migrate mainly to in skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3 delta are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3 delta share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3 delta. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma delta T cells, were used as the immunogen for the GL-3/5E11 antibody(1).

References

Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.

Alternate Names

delta polypeptide, T3D, TiT3

Entrez Gene IDs

915 (Human); 12500 (Mouse); 25710 (Rat)

Gene Symbol

CD3D

Additional TCR gamma/delta Products

Product Documents for Mouse TCR gamma/delta Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TCR gamma/delta Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TCR gamma/delta Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7297

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TCR gamma/delta Antibody

Detection of TCR gamma/ delta in Mouse Splenocytes by Flow Cytometry.