Mouse TER-119 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1125
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
CyTOF-reported, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # TER-119
Product Specifications
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Mouse TER-119 Antibody
Application
Recommended Usage
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse bone marrow
Sample: Mouse bone marrow
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic liver (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryonic liver (13 d.p.c.)
Immunoprecipitation
Kina, T. et al. (2000) Br. J. Haematol. 109:280
Western Blot
Kina, T. et al. (2000) Br. J. Haematol. 109:280
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB1125 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TER-119
Additional TER-119 Products
Product Documents for Mouse TER-119 Antibody
Product Specific Notices for Mouse TER-119 Antibody
For research use only
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