Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # TER-119

View all TER-119 Antibodies »

Product Specifications

Immunogen

C57BL/6 mouse day-14 fetal liver cells

Specificity

Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, and adult peripheral blood.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse TER-119 Fluorescein-conjugated Antibody

Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (FAB1217P) and either (A) Rat Anti-Mouse TER-119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1125F) or (B) Rat IgG2BFluorescein Isotype Control (IC013F). View our protocol for Staining Membrane-associated Proteins.
Detection of TER-119 antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry.

Detection of TER-119 in Mouse Bone Marrow Cells by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER-119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1125F) or (B) Rat IgG2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TER-119 Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse bone marrow cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TER-119

The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).

References

  1. Kina, T. et al. (2000) Br. J. Haematol. 109:280.
  2. Ikuta, K. et al. (1990) Cell 62:863.
  3. Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5

Alternate Names

TER119

Entrez Gene IDs

104231 (Mouse)

Gene Symbol

LY76

Additional TER-119 Products

Product Documents for Mouse TER-119 Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TER-119 Fluorescein-conjugated Antibody

For research use only

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