Mouse TGF-beta RII Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB532
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 129502
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TGF-beta RII
Ile24-Asp184
Accession # Q62312
Ile24-Asp184
Accession # Q62312
Specificity
Detects mouse TGF-beta RII in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with with recombinant rat MIS RII, recombinant mouse TGF-beta RI, recombinant human (rh) TGF-beta RII, rhTGF-beta RIIb, or rhTGF-beta RIII.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse TGF-beta RII Antibody
Detection of Mouse TGF-beta RII by Western Blot
Differential Role of Cathepsins in UUO-Induced Kidney Fibrosis(A) Immunofluorescent staining of epithelial cell marker ZO-1 (green) with nuclei counterstained with DAPI (blue) in kidneys from sham WT or 14 days post-UUO WT, Ctsb−/−, Ctsl−/−, Ctss−/−, and Ctsk−/− mice and quantification of positive areas.(B) Immunoblot analysis of TGFBR1, TGFBR2, pSmad-2, and pSmad-3 and quantification relative to GAPDH in kidney extracts from sham WT or 14 days post-UUO WT, Ctsb−/−, and Ctsl−/− mice.(C) Immunoblot analysis of TGFBR1, TGFBR2, pSmad-2, and pSmad-3 and quantification relative to GAPDH in kidney extracts from sham WT or 14 days post-UUO WT, Ctss−/−, and Ctsk−/− mice.(D) Immunostaining of pSmad-2 and pSmad-3 in kidneys from the same sets of mice and quantification of positive areas.Scale in A: 100 μm. Scale in D: 50 μm. n = 8–10 per group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31446224), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse TGF-beta RII Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse TGF-beta RII Fc Chimera (Catalog # 1600-R2)
Sample: Recombinant Mouse TGF-beta RII Fc Chimera (Catalog # 1600-R2)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 3 using MAB532 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta RII
Long Name
Transforming Growth Factor beta Receptor II
Alternate Names
TGF-bRII, TGFbetaRII, TGFBR2
Gene Symbol
TGFBR2
UniProt
Additional TGF-beta RII Products
Product Documents for Mouse TGF-beta RII Antibody
Product Specific Notices for Mouse TGF-beta RII Antibody
For research use only
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