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Mouse TIGIT Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7267G

R&D Systems, part of Bio-Techne
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FAB7267G

Key Product Details

Validated by

Biological Validation

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIGIT
Gly26-Thr143
Accession # NP_001139797

Specificity

Detects mouse TIGIT in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human TIGIT is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse TIGIT Alexa Fluor® 488-conjugated Antibody

Detection of TIGIT antibody in Mouse splenocytes antibody by Flow Cytometry.

Detection of TIGIT in Mouse splenocytes by Flow Cytometry.

Mouse splenocytes treated with 50 ng/mL PMA and 200 ng/mL Calcium Ionomycin overnight were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Sheep Anti-Mouse TIGIT Alexa Fluor® 488-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7267G) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse TIGIT Alexa Fluor® 488-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: Mouse splenocytes treated with PMA and calcium ionomycin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to either PVR/CD155 or PVRL2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, TIGIT and CD226/DNAM1 pairings with PVR and PVRL2 appear to form a network that resembles the well-characterized B7-1 and B7-2 pairings with CD28 and CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity and IFN-gamma production. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is unusual in that it shows an addition of nine amino acids spread over three insertion sites. Over aa 26-143, mouse TIGIT shares only 84% aa sequence identity with rat TIGIT, but only 68% aa sequence identity with human TIGIT.

Long Name

T Cell Immunoreceptor with Ig and ITIM Domains

Alternate Names

VSIG9, VSTM3, WUCAM

Entrez Gene IDs

201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

Gene Symbol

TIGIT

UniProt

Additional TIGIT Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse TIGIT Alexa Fluor® 488-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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