Mouse TNF-alpha Antibody

Detection of Recombinant Mouse, and Rat TNF‑ alpha by Western Blot.

Western blot shows 25 ng of Recombinant Mouse TNF-a aa 80-235 (Catalog # 410-MT), Recombinant Human TNF-a (Catalog # 210-TA), Recombinant Rat TNF-a (Catalog # 510-RT) and Recombinant Mouse Lymphotoxin- a/TNF- beta. PVDF Membrane was probed with 1 µg/mL of Hamster Anti-Mouse TNF-a Monoclonal Antibody (Catalog # MAB410) followed by HRP-conjugated Anti-Hamster IgG Secondary Antibody. A specific band was detected for TNF-a at approximately 15 kDa (as indicated). This experiment was conducted under reducing and non reducing conditions using Immunoblot Buffer Group 3.

Detection of Mouse TNF-alpha by Immunohistochemistry

Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.