Mouse TNF-alpha Antibody

Cytotoxicity Induced by TNF-alpha and Neutralization by Mouse TNF-alpha Antibody.

Recombinant Mouse TNF-a (Catalog # 410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF-a Polyclonal Antibody (Catalog # AB-410-NA). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Detection of Mouse Mouse TNF-alpha Antibody by Western Blot

Effect of a TNF-alpha -neutralizing antibody on LPS-induced perturbation of muscle differentiation.(A) C2C12 myoblasts were cultured for 48 h in DM alone, LPS (1 μg/mL), or LPS (1 μg/mL) plus TNF-alpha -neutralizing antibody (5 μg/mL). A representative western blot probed with antibodies to myogenin, MyoD, or beta-tubulin (internal standard) is shown. (B and C) Quantification of the data presented in (A). Data are the mean ± SEM of 7–15 independent experiments. (D) C2C12 myoblasts were cultured for 144 h as described in (A). A representative western blot probed with antibodies to MyHC II, myostatin, or beta-tubulin (internal standard) is shown. (E and F) Quantification of the data presented in (D). Data are the mean ± SEM of 5–21 independent experiments. (G) Cells were treated as described in (A), and NF-kappa B activity was analyzed by ELISA. Data are the mean ± SEM of 7 independent experiments performed in duplicate. ***p < 0.001, **p < 0.01, *p < 0.05, ###p < 0.001, ##p < 0.01, #p < 0.05 by one-way ANOVA followed by Tukey’s honest significant difference test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28742154), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Mouse TNF-alpha Antibody by Western Blot

Effect of a TNF-alpha -neutralizing antibody on LPS-induced perturbation of muscle differentiation.(A) C2C12 myoblasts were cultured for 48 h in DM alone, LPS (1 μg/mL), or LPS (1 μg/mL) plus TNF-alpha -neutralizing antibody (5 μg/mL). A representative western blot probed with antibodies to myogenin, MyoD, or beta-tubulin (internal standard) is shown. (B and C) Quantification of the data presented in (A). Data are the mean ± SEM of 7–15 independent experiments. (D) C2C12 myoblasts were cultured for 144 h as described in (A). A representative western blot probed with antibodies to MyHC II, myostatin, or beta-tubulin (internal standard) is shown. (E and F) Quantification of the data presented in (D). Data are the mean ± SEM of 5–21 independent experiments. (G) Cells were treated as described in (A), and NF-kappa B activity was analyzed by ELISA. Data are the mean ± SEM of 7 independent experiments performed in duplicate. ***p < 0.001, **p < 0.01, *p < 0.05, ###p < 0.001, ##p < 0.01, #p < 0.05 by one-way ANOVA followed by Tukey’s honest significant difference test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28742154), licensed under a CC-BY license. Not internally tested by R&D Systems.