Mouse TORC2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6458
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 632307
Product Summary for Mouse TORC2 Antibody
Immunogen
E. coli-derived recombinant mouse TORC2
Lys454-Ser612
Accession # Q3U182
Lys454-Ser612
Accession # Q3U182
Specificity
Detects mouse TORC2 in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse TORC2 Antibody
Detection of Mouse TORC2 by Western Blot.
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 2 µg/mL of Mouse TORC2 Polyclonal Antibody (Catalog # MAB6458) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TORC2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Mouse TORC2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line
Sample: NIH‑3T3 mouse embryonic fibroblast cell line, DA3 mouse myeloma cell line, and RAW 264.7 mouse monocyte/macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TORC2
TORC2, also known as CREB-regulated transcription coactivator 1 (crtc2), is a 70-80 kDa protein that functions as a coactivator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 454-612 , mouse TORC2 shares 84% and 98% aa sequence identity with human and rat TORC2, respectively.
Long Name
Transducer of Regulated CREB protein 1
Alternate Names
CRTC2
Gene Symbol
CRTC2
UniProt
Additional TORC2 Products
Product Documents for Mouse TORC2 Antibody
Product Specific Notices for Mouse TORC2 Antibody
For research use only
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