Mouse TRAIL R2/TNFRSF10B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1540
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 118929
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRAIL R2/TNFRSF10B
Asn53-Ser177 (predicted)
Accession # Q9QZM4
Asn53-Ser177 (predicted)
Accession # Q9QZM4
Specificity
Detects mouse TRAIL R2/TNFRSF10B in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse TRAIL R2/TNFRSF10B Antibody
TRAIL R2/TNFRSF10B in Mouse Brain.
TRAIL R2/TNFRSF10B was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Rat Anti-Mouse TRAIL R2/TNFRSF10B Monoclonal Antibody (Catalog # MAB1540) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of Purkinje neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse TRAIL R2/TNFRSF10B Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)
Western Blot
1 µg/mL
Sample: Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 721-DR)
Sample: Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera (Catalog # 721-DR)
Reviewed Applications
Read 1 review rated 5 using MAB1540 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B
Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid (aa) precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% aa sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.
References
- Chaudhary, P.M. et al. (1997) Immunity 7:821.
- Walczak, H. et al. (1997) EMBO J. 16:5386.
- Golstein, P. (1997) Curr. Biol. 7:R750.
- Wu, G.S. et al. (1999) Cancer Research 59:2770.
Long Name
TRAIL Receptor 2
Alternate Names
CD262, DR5, TNFRSF10B, TRAILR2
Gene Symbol
TNFRSF10B
UniProt
Additional TRAIL R2/TNFRSF10B Products
Product Documents for Mouse TRAIL R2/TNFRSF10B Antibody
Product Specific Notices for Mouse TRAIL R2/TNFRSF10B Antibody
For research use only
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