CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524, filled histo-gram) or isotype control antibody (Catalog # MAB0061, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

TSPAN8 in Mouse Intestine.

TSPAN8 was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse TSPAN8 Monoclonal Antibody (Catalog # MAB6524) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to membranes of epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse TSPAN8 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: CMT‑93 mouse rectal carcinoma cell line

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSPAN8

Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.

Long Name

Tetraspanin 8/Transmembrane 4 Superfamily, Member 3

Alternate Names

CO-029, TM4SF3

Entrez Gene IDs

7103 (Human); 216350 (Mouse); 171048 (Rat)

Gene Symbol

TSPAN8

Additional TSPAN8 Products

Product Documents for Mouse TSPAN8 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse TSPAN8 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse TSPAN8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6524

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all TSPAN8 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse TSPAN8 Antibody

Detection of TSPAN8 in CMT-93 Mouse Cell Line by Flow Cytometry.