Mouse Tyro3/Dtk Antibody
R&D Systems, part of Bio-Techne | Catalog # AF759
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
Ala31-Ser418
Accession # P55144
Ala31-Ser418
Accession # P55144
Specificity
Detects mouse Tyro3/Dtk in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Tyro3/Dtk Antibody
Tyro3/Dtk in Mouse Embryo.
Tyro3/Dtk was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c, follicles of vibrissae associated with upper lip) using Goat Anti-Mouse Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF759) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to the follicles of vibrissa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Tyro3/Dtk Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c, follicles of vibrissae associated with upper lip)
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c, follicles of vibrissae associated with upper lip)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)
Sample: Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tyro3/Dtk
Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.
References
- Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
- Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Alternate Names
Brt, Dtk, Rse, Sky
Gene Symbol
TYRO3
UniProt
Additional Tyro3/Dtk Products
Product Documents for Mouse Tyro3/Dtk Antibody
Product Specific Notices for Mouse Tyro3/Dtk Antibody
For research use only
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