Mouse VCAM-1/CD106 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6434
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 112702
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VCAM-1
Phe25-Glu698
Accession # P29533
Phe25-Glu698
Accession # P29533
Specificity
Detects mouse VCAM-1 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) VCAM-1, rmICAM-1, rhICAM-2, rmICAM-2, rhICAM-3, or rhPECAM.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse VCAM-1/CD106 Antibody
Detection of Mouse VCAM‑1/CD106 by Western Blot.
Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6434) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VCAM-1/ CD106 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse VCAM‑1/CD106 by Simple WesternTM.
Simple Western lane view shows lysate of 3T3-L1 mouse embryonic fibroblast adipose-like cell line, loaded at 0.2 mg/mL. A specific band was detected for VCAM-1/CD106 at approximately 117 kDa (as indicated) using 100 µg/mL of Rat Anti-Mouse VCAM-1/CD106 Monoclonal Antibody (Catalog # MAB6434) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse VCAM-1/CD106 by Western Blot
Effect of statin treatment on aortic expression of VCAM-1.(A) VCAM-1 protein expression in the ascending aorta assessed by Western blot in non-treated (lanes 1–5) versus statin treated (lanes 6–10) animals, n = 5 per group, *p<0.01 vs non-treated animals. Representative examples of fluorescent immunohistochemistry images of the base of the aorta demonstrating endothelial VCAM-1 expression (red fluorescence) in a non-treated animal (B) and a statin treated animal (C), the green fluorescence is autofluorescence delineating vessel anatomy. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23554922), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse VCAM-1/CD106 Antibody
Application
Recommended Usage
Simple Western
100 µg/mL
Sample: C2C12 mouse myoblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: C2C12 mouse myoblast cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Western Blot
2 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VCAM-1/CD106
VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha, and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha4 beta7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.
During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha4 beta7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.
ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.
Long Name
Vascular Cell Adhesion Molecule 1
Alternate Names
CD106, VCAM1
Gene Symbol
VCAM1
UniProt
Additional VCAM-1/CD106 Products
Product Documents for Mouse VCAM-1/CD106 Antibody
Product Specific Notices for Mouse VCAM-1/CD106 Antibody
For research use only
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