Mouse VEGF‑B167 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2595

R&D Systems, part of Bio-Techne
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MAB2595
MAB2595-SP
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 128313

View all VEGF-B Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse VEGF-B167
Pro22-Gly171
Accession # P49766-2

Specificity

Detects mouse VEGF-B167 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF-B167, recombinant mouse (rm) VEGF-B186, and rmVEGF-D is observed but no cross-reactivity with rmVEGF165 or rhVEGF-C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse VEGF‑B167 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse VEGF-B167 (Catalog # 2595-VE)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B; also known as VFR) is a member of the VEGF-PDGF supergene family of growth factor molecules (1 - 4). Five mouse members have been identified, including VEGF-A, -B, -C, -D, and PlGF(-2) (1, 5). VEGF family members are disulfide-linked homo- and heterodimeric proteins that are important regulators of vasculogenesis and lymphangiogenesis. Mouse VEGF-B has two isoforms, a 32 kDa single chain and a 21 kDa single chain form (6, 7). The long form (VEGF-B186) is 207 amino acids (aa) in length, with a 21 aa signal sequence and a 186 aa mature region. The short form (VEGF-B167) is 188 aa in length, with a 21 aa signal sequence and a 167 aa mature segment. Each mature isoform shows the same N-terminal 94 aa that contains a cysteine knot VEGF homology domain (6 - 8). VEGF-B186 is O-glycosylated; VEGF-B167 is not. VEGF-B167 binds heparin; VEGF-B186 does not. Thus, VEGF-B186 is secreted and freely diffusible in tissues (7). However, the VEGF-B167 isoform is the predominant form in tissue (9). Mouse VEGF-B186 is 93% and 87% aa identical to bovine and human VEGF-B186, respectively; mouse VEGF-B167 is 90% and 88% aa identical to bovine and human VEGF-B167, respectively . The mouse VEGF-B167 homodimer is 42 kDa in size, while the VEGF-B186 homodimer is 62 kDa in size. Unlike VEGF167, VEGF-B186 undergoes proteolytic processing that creates a partially processed 48 kDa homodimer and a fully processed 32 kDa homodimer. Processing appears to occur at Arg127 of the mature form (10). Both forms of VEGF-B can heterodimerize with VEGF (7). Both VEGF-B isoforms bind to VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (11). VEGF-B167 also binds neuropilin-1, but only the 127 aa processed form of VEGF-B186 binds neuropilin-1 (10). As a dimer, full length VEGF-B186 does not interact with neuropilin-1, while any dimer that contains the processed VEGF-B127 subunit will interact with neuropilin-1 (10). The importance of differential neuropilin binding is unclear. VEGF-B deficient mice display an atrial conduction deficit (12). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1 (11).

References

  1. Li, X. and U. Eriksson (2001) Int. J. Biochem Cell Biol. 33:421.
  2. Olofsson, B. et al. (1999) Curr. Opin. Biotechnol. 10:528.
  3. Clauss, M. (2000) Semin. Thromb. Hemost. 26:561.
  4. Matsumoto, T. and L. Claesson-Welsh (2001) Sci STKE Dec. 11(112):RE21.
  5. DiPalma, T. et al. (1996) Mamm. Genome 7:6.
  6. Olofsson, B. et al. (1996) Proc. Natl. Acad. Sci. USA 93:2576.
  7. Olofsson, B. et al. (1996) J. Biol. Chem. 271:19310.
  8. Twonson, S. et al. (1996) Biochem. Biophys. Res. Commun. 220:922.
  9. Li, X. et al. (2001) Growth Factors 19:49.
  10. Makinen, T. et al. (1999) J. Biol. Chem. 274:21217.
  11. Olofsson, B. et al. (1998) Proc. Nat. Acad. Sci. USA 95:11709.
  12. Aase, K. et al. (2001) Circulation 104:358.

Long Name

Vascular Endothelial Growth Factor B

Alternate Names

VEGFB

Entrez Gene IDs

7423 (Human); 22339 (Mouse)

Gene Symbol

VEGFB

UniProt

P49766-2

Additional VEGF-B Products

Product Documents for Mouse VEGF‑B167 Antibody

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Product Specific Notices for Mouse VEGF‑B167 Antibody

For research use only

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