Mouse Vitronectin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB38751
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry
Cited:
Bioassay, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 347317
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Vitronectin
Asp20-Lys478
Accession # AAH12690
Asp20-Lys478
Accession # AAH12690
Specificity
Detects mouse Vitronectin in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Vitronectin is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Vitronectin Antibody
Vitronectin in Mouse Brain.
Vitronectin was detected in immersion fixed frozen sections of fetal mouse brain using 10 µg/mL Rat Anti-Mouse Vitronectin Monoclonal Antibody (Catalog # MAB38751) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse Vitronectin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of fetal mouse brain
Sample: Immersion fixed frozen sections of fetal mouse brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Vitronectin
Vitronectin is a 71 kDa secreted glycoprotein produced by the liver and tumor cells. In blood, Vitronectin is called serum spreading factor. In the extracellular matrix, its function is determined by binding partners such as PAI-1, complement factors, integrins (notably alphav beta3) and thrombin. The 459 amino acid (aa) mature mouse Vitronectin shows 74% aa sequence identity with human Vitronectin and contains somatomedin B-like and hemopexin-like domains, an RGD motif, a basic heparin-binding domain and sulfated tyrosines. Unbound Vitronectin is a monomer that may be cleaved to form a dimer of 65 kDa and 10 kDa components.
Alternate Names
Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN
Gene Symbol
VTN
UniProt
Additional Vitronectin Products
Product Documents for Mouse Vitronectin Antibody
Product Specific Notices for Mouse Vitronectin Antibody
For research use only
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