Mouse Wnt-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1620
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse Wnt-1 peptide
Ser52-Glu92 and Met234-Arg313
Accession # Q3UR96
Ser52-Glu92 and Met234-Arg313
Accession # Q3UR96
Specificity
Detects mouse Wnt-1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑3a and rmWnt‑5a is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Wnt-1 Antibody
Wnt-1 in Mouse Embryonic Spinal Cord.
Wnt-1 was detected in immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Wnt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1620) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Wnt-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.)
Sample: Immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Wnt-1
Sample: Recombinant Mouse Wnt-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-1
Long Name
Wingless-type MMTV Integration Site Family, Member 1
Alternate Names
Int-1, Wnt1
Gene Symbol
WNT1
UniProt
Additional Wnt-1 Products
Product Documents for Mouse Wnt-1 Antibody
Product Specific Notices for Mouse Wnt-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...