Mouse Wnt-9b Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB36691

R&D Systems, part of Bio-Techne
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MAB36691-100
MAB36691-SP
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Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 964742

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Product Summary for Mouse Wnt-9b Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-9b
Met1-Arg359
Accession # O35468

Specificity

Detects mouse Wnt-9b in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Wnt-9b Antibody

Alkaline Phosphatase Production Induced by Wnt‑9b and Neutralization by Mouse Wnt‑9b Antibody.

Alkaline Phosphatase Production Induced by Wnt‑9b and Neutralization by Mouse Wnt‑9b Antibody.

Recombinant Mouse Wnt-9b (Catalog # 3669-WN) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse Wnt-9b (400 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Wnt-9b Monoclonal Antibody (Catalog # MAB36691). The ND50 is typically 125-750 ng/mL.

Applications for Mouse Wnt-9b Antibody

Application
Recommended Usage

Neutralization

Measured by its ability to neutralize Wnt‑9b-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 125-750 ng/mL in the presence of 400 ng/mL Recombinant Mouse Wnt‑9b.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-9b

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta-catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

References

  1. http://www.stanford.edu/group/nusselab/cgi-bin/wnt/.
  2. Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
  3. Kirikoshi, H. and  M. Katoh (2002) Int. J. Mol. Med. 9:135.
  4. Qian, J. et al. (2003) Genomics 81:34.
  5. Carroll, T. J. et al. (2005) Dev. Cell 9:283.
  6. Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
  7. Park, J-S. et al. (2007) Development 134:2533.
  8. Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
  9. Juriloff, D. M. and  M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.

Long Name

Wingless-type MMTV Integration Site Family, Member 9b

Alternate Names

Wnt-14b, Wnt-15, Wnt9b

Entrez Gene IDs

7484 (Human); 22412 (Mouse); 303586 (Rat)

Gene Symbol

WNT9B

UniProt

O35468 (Mouse)

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Product Documents for Mouse Wnt-9b Antibody

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Certificate of Analysis

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Product Specific Notices for Mouse Wnt-9b Antibody

For research use only

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