Mouse YM1/Chitinase 3-like 3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2446
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3
Tyr22-Tyr398
Accession # O35744
Tyr22-Tyr398
Accession # O35744
Specificity
Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse YM1/Chitinase 3-like 3 Antibody
Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.
Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse YM1/Chitinase 3‑like 3 by Simple WesternTM.
Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3-like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Mouse YM1/Chitinase 3-like 3 Antibody
Application
Recommended Usage
Simple Western
2.5 µg/mL
Sample: Mouse bone marrow
Sample: Mouse bone marrow
Western Blot
0.2 µg/mL
Sample: Mouse bone marrow
Sample: Mouse bone marrow
Reviewed Applications
Read 1 review rated 5 using AF2446 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: YM1/Chitinase 3-like 3
Alternate Names
CHI3L3, Chitinase 3 like 3, Chitinase 3-like 3, ECF-L
Entrez Gene IDs
12655 (Mouse)
Gene Symbol
CHIL3
UniProt
Additional YM1/Chitinase 3-like 3 Products
Product Documents for Mouse YM1/Chitinase 3-like 3 Antibody
Product Specific Notices for Mouse YM1/Chitinase 3-like 3 Antibody
For research use only
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