MRG15 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1307
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence APKQDPKPKFQEG-C corresponding to N-Terminus according to NP_006782.1, NP_996670.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_006782.1) and isoform 2 (NP_996670.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for MRG15 Antibody
![Western Blot: MRG15 Antibody [NB100-1307]](https://resources.bio-techne.com/images/products/MRG15-Antibody-Western-Blot-NB100-1307-img0006.jpg "Western Blot: MRG15 Antibody [NB100-1307]")
Western Blot: MRG15 Antibody [NB100-1307]
Western Blot: MRG15 Antibody [NB100-1307] - staining of NIH3T3 (A) and HepG2 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence![Western Blot: MRG15 Antibody [NB100-1307]](https://resources.bio-techne.com/images/products/MRG15-Antibody-Western-Blot-NB100-1307-img0007.jpg "Western Blot: MRG15 Antibody [NB100-1307]")
Western Blot: MRG15 Antibody [NB100-1307]
Western Blot: MRG15 Antibody [NB100-1307] - staining of Mouse Liver (A), Rat Liver (B) and Pig Liver (C) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] -
Immunohistochemistry-Paraffin: MRG15 Antibody [NB100-1307] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Applications for MRG15 Antibody
Application
Recommended Usage
Immunohistochemistry
4-6 ug/ml
Immunohistochemistry-Paraffin
4-6 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.1-0.3 ug/ml
Application Notes
Western blot: Approx 37kDa band observed in nuclear lysates of cell lines HepG2 and NIH3T3 and approx 40kDa band observed in Mouse, Rat and Pig Liver lysates (calculated MW of 37.2kDa according to Human NP_006782.1, Mouse NP_077751.1, Rat NP_001011999.1 and Pig XP_001927776.1 and 41.5kDa according to Human NP_996670.1, Mouse NP_001034236.1 and Pig XP_001927789.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MRG15
Alternate Names
Eaf3, Esa1p-associated factor 3 homolog, HsT17725, MEAF3, MGC10631, MORF-related gene on chromosome 15, MORFRG15, mortality factor 4 like 1, mortality factor 4-like protein 1, MRG15MORF-related gene 15 protein, Protein MSL3-1, S863-6, Transcription factor-like protein MRG15
Gene Symbol
MORF4L1
UniProt
Additional MRG15 Products
Product Documents for MRG15 Antibody
Product Specific Notices for MRG15 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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