MRPL45 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82764

Novus Biologicals, part of Bio-Techne
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NBP1-82764
NBP1-82764-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MRPL45 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLEYVVFEKQLTNPYGSWRMHTKIVPPWAPPKQPILKTVMIPGPQLKPEEEYEEAQGEAQKPQLA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MRPL45 Antibody

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764]

Immunohistochemistry-Paraffin: MRPL45 Antibody [NBP1-82764] - Staining of human kidney, liver, prostate and small intestine using Anti-MRPL45 antibody NBP1-82764 (A) shows similar protein distribution across tissues to independent antibody NBP1-82763 (B).
Western Blot: MRPL45 Antibody [NBP1-82764]

Western Blot: MRPL45 Antibody [NBP1-82764]

Western Blot: MRPL45 Antibody [NBP1-82764] - Analysis using Anti-MRPL45 antibody NBP1-82764 (A) shows similar pattern to independent antibody NBP1-82763 (B).
Immunocytochemistry/ Immunofluorescence: MRPL45 Antibody [NBP1-82764]

Immunocytochemistry/ Immunofluorescence: MRPL45 Antibody [NBP1-82764]

Immunocytochemistry/Immunofluorescence: MRPL45 Antibody [NBP1-82764] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
View 4 more images

Applications for MRPL45 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MRPL45

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within themitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. Theyhave an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes inbiochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.Pseudogenes corresponding to this gene are found on chromosomes 2p and 17q. (provided by RefSeq)

Alternate Names

L45mt, MGC11321, mitochondrial ribosomal protein L45, MRP-L45,39S ribosomal protein L45, mitochondrial

Gene Symbol

MRPL45

Additional MRPL45 Products

Product Documents for MRPL45 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MRPL45 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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