MRRF Antibody (1T5W1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16134
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1T5W1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MRRF Antibody (1T5W1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 163-262 of human MRRF (Q96E11). RESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MRRF Antibody (1T5W1)
Western Blot: MRRF Antibody (1T5W1) [NBP3-16134]
Western Blot: MRRF Antibody (1T5W1) [NBP3-16134] - Western blot analysis of extracts of various cell lines, using MRRF antibody (NBP3-16134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western Blot: MRRF Antibody (1T5W1) [NBP3-16134]
Western Blot: MRRF Antibody (1T5W1) [NBP3-16134] - Western blot analysis of extracts of 293T cells, using MRRF antibody (NBP3-16134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Applications for MRRF Antibody (1T5W1)
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MRRF
Alternate Names
mitochondrial, mitochondrial ribosome recycling factor, MTRRF
Gene Symbol
MRRF
Additional MRRF Products
Product Documents for MRRF Antibody (1T5W1)
Product Specific Notices for MRRF Antibody (1T5W1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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