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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1360) of human MutS homolog 6 using the numbering given in entry NP_000170.1 (GeneID 2956).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MSH6 Antibody - BSA Free

Western Blot: MSH6 Antibody [NB100-329]

Western Blot: MSH6 Antibody [NB100-329]

Western Blot: MSH6 Antibody [NB100-329] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH6 antibody NB100-329 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-329]

Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-329]

Immunohistochemistry-Paraffin: MSH6 Antibody [NB100-329] - Section of human testicular seminoma.Antibody: Affinity purified rabbit anti- MSH6used at a dilution of 1:500 (2 ug/ml). Detection: DAB

Applications for MSH6 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:250-1:1000

Western Blot

1:1000-1:5000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MSH6

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Alternate Names

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBPHNPCC5, GTMBP, hMSH6, HSAP, mutS (E. coli) homolog 6, mutS homolog 6 (E. coli), MutS-alpha 160 kDa subunit, p160, sperm-associated protein

Entrez Gene IDs

2956 (Human)

Gene Symbol

MSH6

Additional MSH6 Products

Product Documents for MSH6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MSH6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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