Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4G10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MST2/STK3 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human STK3 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free

Western Blot: MST2/STK3 Antibody (OTI4G10)Azide and BSA Free [NBP2-72794]

Western Blot: MST2/STK3 Antibody (OTI4G10)Azide and BSA Free [NBP2-72794]

Western Blot: MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free [NBP2-72794] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MST2.
Immunohistochemistry: MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free [NBP2-72794]

Immunohistochemistry: MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free [NBP2-72794]

Immunohistochemistry: MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free [NBP2-72794] - Staining of paraffin-embedded lung tissue using anti-MST2 mouse monoclonal antibody.
Western Blot: MST2/STK3 Antibody (OTI4G10)Azide and BSA Free [NBP2-72794]

Western Blot: MST2/STK3 Antibody (OTI4G10)Azide and BSA Free [NBP2-72794]

Western Blot: MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free [NBP2-72794] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MST2 monoclonal antibody.
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Applications for MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Mammalian Sterile 20-Like 2 (Mst2) is most similar to the previously identified Mst1 protein kinase (78% identity, 88% similarity). Northern analysis indicates that MST2 mRNA is expressed at high levels in adult kidney, skeletal and placental tissues and at very low levels in adult heart, lung, liver and brain tissues. An in-vitro kinase assay indicates that Mst2 can phosphorylate an exogenous substrate, as well as itself, and phospho-amino-acid analysis indicates that it is a serine/threonine protein kinase (1) Mst2, a STE20-family member and purported Hpo ortholog, phosphorylates and activates both Lats1 and Lats2. Results indicate that Mst2-like kinases regulate Lats kinase activities in an evolutionarily conserved regulatory pathway (2). When stably expressed in HeLa cells, Mst highly sensitizes the cells to death receptor-mediated apoptosis by accelerating caspase-3 activation. These findings suggest that Mst1 and Mst2 play a role in apoptosis both upstream and downstream of caspase activation (3).

Alternate Names

KRS1, Mess1, MST3, STK3

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MST2/STK3 Antibody (OTI4G10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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