MST2/STK3 Antibody (OTI4G10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48017

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4G10

Concentration

1 mg/ml

View all MST2/STK3 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human STK3 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens serine/threonine kinase 3 (STE20 homolog, yeast) (STK3).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

56.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MST2/STK3 Antibody (OTI4G10)

Western Blot: MST2/STK3 Antibody (OTI4G10) [NBP1-48017]

Western Blot: MST2/STK3 Antibody (OTI4G10) [NBP1-48017]

Western Blot: MST2/STK3 Antibody (4G10) [NBP1-48017] - MST2 Antibody (4G10) [NBP1-48017] Analysis of extracts (35ug) from 9 different cell lines by using anti-MST2 monoclonal antibody.
Immunohistochemistry-Paraffin: MST2/STK3 Antibody (OTI4G10) [NBP1-48017]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody (OTI4G10) [NBP1-48017]

Immunohistochemistry-Paraffin: MST2/STK3 Antibody (4G10) [NBP1-48017] - Staining of paraffin-embedded Ovary tissue using anti-MST2 mouse monoclonal antibody.
Western Blot: MST2/STK3 Antibody (OTI4G10) [NBP1-48017]

Western Blot: MST2/STK3 Antibody (OTI4G10) [NBP1-48017]

Western Blot: MST2/STK3 Antibody (4G10) [NBP1-48017] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MST2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MST2.
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Applications for MST2/STK3 Antibody (OTI4G10)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MST2/STK3

Protein kinase activation is a frequent response of cells to treatment with growth factors,chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast ' sterile 20'(Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand

Alternate Names

KRS1, Mess1, MST3, STK3

Entrez Gene IDs

6788 (Human)

Gene Symbol

STK3

Additional MST2/STK3 Products

Product Documents for MST2/STK3 Antibody (OTI4G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MST2/STK3 Antibody (OTI4G10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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