MTH1 Antibody [Janelia Fluor® 525]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-109JF525

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB100-109AF350

Alexa Fluor 405

NB100-109AF405

Alexa Fluor 488

NB100-109AF488

Alexa Fluor 532

NB100-109AF532

Alexa Fluor 594

NB100-109AF594

Alexa Fluor 647

NB100-109AF647

Alexa Fluor 700

NB100-109AF700

Alexa Fluor 750

NB100-109AF750

Allophycocyanin

NB100-109APC

Biotin

NB100-109B

DyLight 350

NB100-109UV

DyLight 405

NB100-109V

DyLight 488

NB100-109G

DyLight 550

NB100-109R

DyLight 594

NB100-109DL594

DyLight 650

NB100-109C

DyLight 680

NB100-109FR

DyLight 755

NB100-109IR

FITC

NB100-109F

HRP

NB100-109H

Janelia Fluor 549

NB100-109JF549

Janelia Fluor 585

NB100-109JF585

Janelia Fluor 635

NB100-109JF635

Janelia Fluor 646

NB100-109JF646

Janelia Fluor 669

NB100-109JF669

PE

NB100-109PE

PerCP

NB100-109PCP

Forumulation

Catalog #

BSA Free

NB100-109

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Knockout Validated, Simple Western, Single Cell Western, Western Blot

Label

Janelia Fluor 525

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MTH1 Antibodies »

Product Summary for MTH1 Antibody [Janelia Fluor® 525]

Immunogen

A C-terminal peptide derived from human MTH1, conjugated to KLH. [UniProt# P36639]

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 12706856).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for MTH1 Antibody [Janelia Fluor® 525]

Application

Recommended Usage

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Single Cell Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MTH1

Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Among the various classes of DNA damage caused by oxygen radicals, an oxidized form of guanine base (8-oxoguanine) appears to be important as it can pair with cytosine and adenine and G:C to T:A transversion mutation occurs. A significant amount of 8-OxoG is formed in the chromosomal DNA of mammalian cells, with most damaged nucleotides excised from the DNA and excreted in the urine. Along with 8-oxoG being present in oxidatively damaged DNA, 8-oxo-deoxyguanosine (8-oxo-dGTP) is formed in the nucleotide pool during normal cellular metabolism and following oxidative stress. The 8-oxo-dGTP nucleotide can be incorporated in DNA during polymerization and can result in a mispairing unless repaired. MTH converts 8-oxo-dGTP in the nucleotide pool to the monophosphate and prevents the misincorporation of 8-oxo-dGTP into DNA (Figure courtesy of Dr. Mark Kelley). MTH also recognizes 8-oxo-rGTP, which could incorporate into RNA during gene transcription leading to missense or nonsense protein production.

Long Name

Oxidized purine nucleoside triphosphate hydrolase

Alternate Names

8-oxo-dGTPase, EC 3.6.1.-, EC 3.6.1.56, Nudix motif 1, NUDT1

Gene Symbol

NUDT1

Additional MTH1 Products

Product Documents for MTH1 Antibody [Janelia Fluor® 525]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for MTH1 Antibody [Janelia Fluor® 525]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews