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MTHFD1L Antibody (1E8)

Novus Biologicals, part of Bio-Techne | Catalog # H00025902-M01

Novus Biologicals, part of Bio-Techne
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H00025902-M01

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MTHFD1L (NP_056255, 801 a.a. ~ 899 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VFKTDTRAEIDLVCELAKRAGAFDAVPCYHWSVGGKGSVDLARAVREAASKRSRFQFLYDVQVPIVDKIRTIAQAVYGAKDIELSPEAQAKIDRYTQQG

Specificity

MTHFD1L - methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for MTHFD1L Antibody (1E8)

Western Blot: MTHFD1L Antibody (1E8) [H00025902-M01]

Western Blot: MTHFD1L Antibody (1E8) [H00025902-M01]

Western Blot: MTHFD1L Antibody (1E8) [H00025902-M01] - MTHFD1L monoclonal antibody (M01), clone 1E8 Analysis of MTHFD1L expression in PC-12.
Sandwich ELISA: MTHFD1L Antibody (1E8) [H00025902-M01] - Detection limit for recombinant GST tagged MTHFD1L is 0.3 ng/ml as a capture antibody.

Applications for MTHFD1L Antibody (1E8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MTHFD1L

One-carbon substituted forms of tetrahydrofolate (THF) are involved in the de novo synthesis of purines and thymidylate and support cellular methylation reactions through the regeneration of methionine from homocysteine. MTHFD1L is an enzyme involved in THF synthesis in mitochondria (Christensen et al., 2005 [PubMed 15611115]).[supplied by OMIM]

Alternate Names

10-formyl-THF synthetase, dJ292B18.2, DKFZP586G1517, EC 6.3.4.3, FLJ21145, Formyltetrahydrofolate synthetase, formyltetrahydrofolate synthetase domain containing 1, FTHFSDC1, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, mitochondrial C1-tetrahydrofolate synthase, mitochondrial C1-tetrahydrofolate synthetase, monofunctional C1-tetrahydrofolate synthase, mitochondrial, MTC1THFS

Entrez Gene IDs

25902 (Human)

Gene Symbol

MTHFD1L

Additional MTHFD1L Products

Product Documents for MTHFD1L Antibody (1E8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MTHFD1L Antibody (1E8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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