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MTHFS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20947

Novus Biologicals, part of Bio-Techne
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NBP1-20947

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence KRCLQHQEVKPYT corresponding to internal region according to NP_006432.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MTHFS Antibody

Western Blot: MTHFS Antibody [NBP1-20947]

Western Blot: MTHFS Antibody [NBP1-20947]

Western Blot: MTHFS Antibody [NBP1-20947] - (0.5ug/ml) Analysis of Human Liver lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947]

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947]

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP1-20947] - Staining of paraffin embedded Human Lung at dilution of 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MTHFS Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1.5 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human liver lysates (calculated MW of 23.3 kDa band according to NP_006432.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MTHFS

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids

Alternate Names

5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolatecyclo-ligase), 5-formyltetrahydrofolate cyclo-ligase, EC 6.3.3.25,10-methenyl-tetrahydrofolate synthetase, FLJ30410, HsT19268, Methenyl-THF synthetase

Entrez Gene IDs

10588 (Human); 107885 (Mouse)

Gene Symbol

MTHFS

Additional MTHFS Products

Product Documents for MTHFS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MTHFS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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