MTHFS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-58656

Novus Biologicals, part of Bio-Techne
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NBP2-58656
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MTHFS Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: AAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKRISIFLSMQDEIETEEIIKDIFQRGKICFIPRYRFQSNHMDMVRIESPEEIS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MTHFS Antibody

Western Blot: MTHFS Antibody [NBP2-58656]

Western Blot: MTHFS Antibody [NBP2-58656]

Western Blot: MTHFS Antibody [NBP2-58656] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunocytochemistry/ Immunofluorescence: MTHFS Antibody [NBP2-58656]

Immunocytochemistry/ Immunofluorescence: MTHFS Antibody [NBP2-58656]

Immunocytochemistry/Immunofluorescence: MTHFS Antibody [NBP2-58656] - Staining of human cell line MCF7 shows localization to cytosol.
Immunohistochemistry-Paraffin: MTHFS Antibody [NBP2-58656]

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP2-58656]

Immunohistochemistry-Paraffin: MTHFS Antibody [NBP2-58656] - Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Applications for MTHFS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MTHFS

Contributes to tetrahydrofolate metabolism. Helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids

Alternate Names

5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolatecyclo-ligase), 5-formyltetrahydrofolate cyclo-ligase, EC 6.3.3.25,10-methenyl-tetrahydrofolate synthetase, FLJ30410, HsT19268, Methenyl-THF synthetase

Gene Symbol

MTHFS

Additional MTHFS Products

Product Documents for MTHFS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MTHFS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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