mtRNA polymerase Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18334

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chimpanzee (100%), Feline (90%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 11 amino acids near the carboxyl terminus of human mtRNA polymerase. The immunogen is located within the last 50 amino acids of mtRNA polymerase.

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine (82%); Monkey (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for mtRNA polymerase Antibody - BSA Free

Western Blot: mtRNA polymerase AntibodyBSA Free [NBP3-18334]

Western Blot: mtRNA polymerase AntibodyBSA Free [NBP3-18334]

Western Blot: mtRNA polymerase Antibody [NBP3-18334] - WB Validation in Human Cell Lines. Loading: 15 ug of lysate. Antibodies: mtRNA polymerase, 2 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody - BSA Free [NBP3-18334]

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody - BSA Free [NBP3-18334]

Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody [NBP3-18334] - Immunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling mtRNA polymerase with NBP3-18334 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody - BSA Free [NBP3-18334]

Immunocytochemistry/ Immunofluorescence: mtRNA polymerase Antibody - BSA Free [NBP3-18334]

Immunocytochemistry/Immunofluorescence: mtRNA polymerase Antibody [NBP3-18334] - Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling mtRNA polymerase with NBP3-18334 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Applications for mtRNA polymerase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Western Blot

2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mtRNA polymerase

POLRMT, also known as DNA-directed RNA polymerase, mitochondrial, is a 246 amino acid protein that is 28 kDa, catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. This protein is currently being studied for research on autosomal dominant progressive external ophthalmoplegia, essential thrombocythemia, ophthalmoplegia, immunodeficiency, and malaria. This protein has also shown to have interactions with TFB1M, NFKB2, ICT1, TFB2M, TFAM, and over 200 other proteins in ATP/ITP metabolism, mitochondrial gene expression, transcription from mitochondrial promoters, transcription, RNA polymerase I, RNA polymerase III, and mitochondrial transcription, gene expression, and mitochondrial transcription initiation pathways.

Long Name

DNA-directed RNA polymerase, mitochondrial

Alternate Names

EC 2.7.7.6, MtRPOL, POLRMT

Gene Symbol

POLRMT

Additional mtRNA polymerase Products

Product Documents for mtRNA polymerase Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for mtRNA polymerase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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