mtTFA Antibody (4H2M9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16154
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4H2M9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for mtTFA Antibody (4H2M9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human mtTFA (NP_003192.1). QDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for mtTFA Antibody (4H2M9)
![Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154]](https://resources.bio-techne.com/images/products/mtTFA-Antibody-8O1U0-Western-Blot-NBP3-16154-img0003.jpg "Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154]")
Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154]
Western Blot: mtTFA Antibody (8O1U0) [NBP3-16154] - Western blot analysis of extracts of various cell lines, using mtTFA Rabbit mAb (NBP3-16154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.![Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154]](https://resources.bio-techne.com/images/products/mtTFA-Antibody-8O1U0-Immunocytochemistry-Immunofluorescence-NBP3-16154-img0002.jpg "Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154]")
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154]
Immunocytochemistry/Immunofluorescence: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunofluorescence analysis of U-2 OS cells using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: mtTFA Antibody (4H2M9) [NBP3-16154]](https://resources.bio-techne.com/images/products/mtTFA-Antibody-8O1U0-Immunohistochemistry-Paraffin-NBP3-16154-img0001.jpg "Immunohistochemistry-Paraffin: mtTFA Antibody (4H2M9) [NBP3-16154]")
Immunohistochemistry-Paraffin: mtTFA Antibody (4H2M9) [NBP3-16154]
Immunohistochemistry-Paraffin: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunohistochemistry of paraffin-embedded human colon using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for mtTFA Antibody (4H2M9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:1000 - 1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: mtTFA
Alternate Names
mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial
Gene Symbol
TFAM
Additional mtTFA Products
Product Documents for mtTFA Antibody (4H2M9)
Product Specific Notices for mtTFA Antibody (4H2M9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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