mtTFA Antibody (4H2M9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16154
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4H2M9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human mtTFA (NP_003192.1). QDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for mtTFA Antibody (4H2M9)
Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154] -
Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154] - Western blot analysis of various lysates, using TmtTFA Rabbit mAb at 1:1200 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] -
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] - Confocal imaging of paraffin-embedded Human colon cancer tissue using mtTFA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] -
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154] - Confocal imaging of paraffin-embedded Human kidney tissue using mtTFA Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Applications for mtTFA Antibody (4H2M9)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:1000 - 1:10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: mtTFA
Alternate Names
mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial
Gene Symbol
TFAM
Additional mtTFA Products
Product Documents for mtTFA Antibody (4H2M9)
Product Specific Notices for mtTFA Antibody (4H2M9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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