mtTFA Antibody (4H2M9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16154

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16154-100ul
NBP3-16154-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4H2M9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all mtTFA Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human mtTFA (NP_003192.1). QDAYRAEWQVYKEEISRFKEQLTPSQIMSLEKEIMDKHLKRKAMTKKKELTLLGKPKRPRSAYNVYVAERFQEAKGDSPQEKLKTVKENWKNLSDSEKELY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for mtTFA Antibody (4H2M9)

Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154]

Western Blot: mtTFA Antibody (4H2M9) [NBP3-16154]

Western Blot: mtTFA Antibody (8O1U0) [NBP3-16154] - Western blot analysis of extracts of various cell lines, using mtTFA Rabbit mAb (NBP3-16154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154]

Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (4H2M9) [NBP3-16154]

Immunocytochemistry/Immunofluorescence: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunofluorescence analysis of U-2 OS cells using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: mtTFA Antibody (4H2M9) [NBP3-16154]

Immunohistochemistry-Paraffin: mtTFA Antibody (4H2M9) [NBP3-16154]

Immunohistochemistry-Paraffin: mtTFA Antibody (8O1U0) [NBP3-16154] - Immunohistochemistry of paraffin-embedded human colon using mtTFA Rabbit mAb (NBP3-16154) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for mtTFA Antibody (4H2M9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:1000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mtTFA

TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.

Alternate Names

mitochondrial transcription factor A, MTTF1, mtTFA, TCF-6, TCF6L1, TCF6L2Mitochondrial transcription factor 1, TCF6TCF6L3, Transcription factor 6, Transcription factor 6-like 2, Transcription factor 6-like 2 (mitochondrial transcription factor), transcription factor A, mitochondrial

Gene Symbol

TFAM

Additional mtTFA Products

Product Documents for mtTFA Antibody (4H2M9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for mtTFA Antibody (4H2M9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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