MUC12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82607

Novus Biologicals, part of Bio-Techne
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NBP1-82607
NBP1-82607-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MUC12 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EELFENLAEIVKAKIMNETRTTLLDPDSCRKAILCYSEEDTFVDSSVTPGFDFQEQCTQKAAEGYTQFYYVDVLDGKLACVNKCTKGTKSQMNCNLGTCQLQRSGPRCLCPNTNTHWYWG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MUC12 Antibody

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607]

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607]

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607] - Staining in human rectum and liver tissues using anti-MUC12 antibody. Corresponding MUC12 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607]

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607]

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607]

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607]

Immunohistochemistry-Paraffin: MUC12 Antibody [NBP1-82607] - Staining of human rectum shows moderate positivity in luminal membrane of glandular cells.
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Applications for MUC12 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MUC12

Human MUC12 (Mucin 12) is a novel mucin of epithelial mucins, which are large, secreted or cell surface glycoproteins involved in epithelial cell protection, adhesion modulation, and signaling. Using differential display on RNA from paired normal colonic mucosa and primary colorectal tumor, MUC12 is identified as one of the 2 partial cDNAs representing novel mucin genes as well as MUC11. They are downregulated in colorectal tumors.The deduced MUC12 protein contains a predicted transmembrane domain, 2 extracellular cysteine-rich EGF-like domains, a coiled-coil region, and a domain consisting of serine-, threonine-, and proline-rich degenerate tandem repeats of 28 amino acids, a structural feature typical of mucins. Human MUC12 shares high sequence homology with MUC3 and MUC4 . Northern blot analysis of 50 different normal tissues detected MUC12 expression in colon, pancreas, prostate, and uterus, with highest expression in colon. The MUC12 transcript is large, estimated to be longer than 12 kb. Expression of MUC12 was downregulated or absent in 6 of 15 (40%) colorectal tumors, as compared with matched normal colonic tissues. MUC12 expression was not detected in any of 6 colorectal cancer cell lines examined. Epitheial mucins are secreted glycoproteins that play roles in cell protection, adhesion modulation and signaling. MUC 12 is a novel mucin gene and may be involved in epithelial cell growth regulation. MUC 12 is down-regulated in colorectal cancers.

Alternate Names

MUC11, MUC-11, MUC-12, mucin 11, mucin 12, cell surface associated, mucin-11, mucin-12

Gene Symbol

MUC12

Additional MUC12 Products

Product Documents for MUC12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MUC12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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