MUC3 Antibody (MUC3/1154)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44434

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # MUC3/1154

Concentration

0.2 mg/ml

View all MUC3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.

Reactivity Notes

Does not react with Rat.

Localization

Cytoplasmic and cell surface

Specificity

It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

1100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47687)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MUC3 Antibody (MUC3/1154)

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434]

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434]

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human colorectal cancer tissue stained with MUC3 Antibody (MUC3/1154). IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434]

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434]

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human Colon Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434]

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434]

Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) [NBP2-44434] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).
View 1 more images

Applications for MUC3 Antibody (MUC3/1154)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-44434 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MUC3

The mucin genes encode epithelial glycoproteins, some of which are secreted and some membrane bound. Each of the genes contains at least one large domain of tandemly repeated sequence that encodes the peptide sequence rich in serine and/or threonine residues, which carries most of the O-linked glycosylation (Gendler and Spicer, 1995 (PubMed 7778880)).(supplied by OMIM)

Alternate Names

MUC-3A, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin-3A

Gene Symbol

MUC3A

UniProt

Q02505 (Human), Q9H195 (Human)

Additional MUC3 Products

Product Documents for MUC3 Antibody (MUC3/1154)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MUC3 Antibody (MUC3/1154)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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