MUC3 Antibody (MUC3/1154) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47687
![Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]](https://resources.bio-techne.com/images/products/MUC3-Antibody-MUC3-1154-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47687-img0003.jpg "Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # MUC3/1154
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for MUC3 Antibody (MUC3/1154) - Azide and BSA Free
Immunogen
A synthetic peptide of 35 amino acids, SIB35 (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS), which contains two of the MUC3 tandem repeats, coupled to KLH.
Reactivity Notes
Does not react with Rat.
Localization
Cytoplasmic and cell surface
Specificity
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This monoclonal antibody shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
1100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44434).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for MUC3 Antibody (MUC3/1154) - Azide and BSA Free
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687] - Human Colon Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).![Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]](https://resources.bio-techne.com/images/products/MUC3-Antibody-MUC3-1154-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47687-img0001.jpg "Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]")
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).![Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]](https://resources.bio-techne.com/images/products/MUC3-Antibody-MUC3-1154-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47687-img0002.jpg "Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]")
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687]
Immunohistochemistry-Paraffin: MUC3 Antibody (MUC3/1154) - Azide and BSA Free [NBP2-47687] - Human Gastric Carcinoma stained with MUC3 Monoclonal Antibody (MUC3/1154).Applications for MUC3 Antibody (MUC3/1154) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MUC3
Alternate Names
MUC-3A, mucin 3, intestinal, mucin 3A, cell surface associated, mucin 3A, intestinal, mucin-3A
Gene Symbol
MUC3A
UniProt
Additional MUC3 Products
Product Documents for MUC3 Antibody (MUC3/1154) - Azide and BSA Free
Product Specific Notices for MUC3 Antibody (MUC3/1154) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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