MUC6 Antibody (CLH5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44374
![Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374]](https://resources.bio-techne.com/images/products/MUC6-Antibody-CLH5-Immunohistochemistry-Paraffin-NBP2-44374-img0003.jpg "Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CLH5
Concentration
0.2 mg/ml
Product Specifications
Immunogen
A synthetic peptide of the Gastric MUC6 tandem repeat sequence (Uniprot: Q6W4X9 )
Localization
Cytoplasmic & Secreted
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
252 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11450)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MUC6 Antibody (CLH5)
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374]
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Formalin-fixed, paraffin-embedded human normal stomach. Strong cytoplasmic of glandular cells using MUC6 Antibody (CLH5). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.![Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374]](https://resources.bio-techne.com/images/products/MUC6-Antibody-CLH5-Immunohistochemistry-Paraffin-NBP2-44374-img0002.jpg "Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374]")
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374]
Immunohistochemistry-Paraffin: MUC6 Antibody (CLH5) [NBP2-44374] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC6 Antibody (CLH5). Courtesy of Dr. Leonor David, IPATIMUP and Medical Faculty, University of Porto, PortugalApplications for MUC6 Antibody (CLH5)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Immunoprecipitation
2-4ug/mg
Western Blot
1:2000
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MUC6
Alternate Names
gastric mucin, Gastric mucin-6, MUC-6, mucin 6, gastric, mucin 6, oligomeric mucus/gel-forming, mucin-6
Gene Symbol
MUC6
UniProt
Additional MUC6 Products
Product Documents for MUC6 Antibody (CLH5)
Product Specific Notices for MUC6 Antibody (CLH5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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