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MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25570

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25570

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1450

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MuRF1/TRIM63. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] -

Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [HL1450] (NBP3-25570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] -

Western Blot: MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free [NBP3-25570] - Human tissue extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with MURF1 antibody [HL1450] (NBP3-25570) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MuRF1/TRIM63

MURF1 encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is localized to the Z-line and M-line lattices of myofibrils, where titin's N-terminal and C-terminal regions respectively bind to the sarcomere. In vitro binding studies have shown that this protein also binds directly to titin near the region of titin containing kinase activity. Another member of this protein family binds to microtubules. Since these family members can form heterodimers, this suggests that these proteins may serve as a link between titin kinase and microtubule-dependent signal pathways in muscle.

Long Name

Tripartite Motif-containing 63

Alternate Names

IRF, RNF28, SMRZ, TRIM63

Gene Symbol

TRIM63

Additional MuRF1/TRIM63 Products

Product Documents for MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MuRF1/TRIM63 Antibody (HL1450) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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