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Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87578

Novus Biologicals, part of Bio-Techne
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NBP1-87578
NBP1-87578-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNPNPSHQMTKRK

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Human reactivity reported in scientific literature (PMID: 24658304).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NBP1-87578]

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NBP1-87578]

Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody [NBP1-87578] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Applications for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 24658304). For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CHRM5

Muscarinic cholinergic receptor M5 is an Acetylcholine Receptor that is active during embryonic development. It is coupled to phosphoinositide degradation and to the activation of mitogen-activated protein kinase (MAPK). The M5 receptor has been reported to be expressed in blood, brain, and ovary. ESTs have been isolated from human testis and placenta libraries.

Long Name

Cholinergic Receptor Muscarinic 5

Alternate Names

HM5, M5, M5R, mAChR M5

Gene Symbol

CHRM5

Additional CHRM5 Products

Product Documents for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Muscarinic Acetylcholine Receptor M5/CHRM5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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