Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F11

Format

BSA Free

Concentration

1 mg/ml

View all Muscle Phosphofructokinase/PFKM Antibodies »

Product Specifications

Immunogen

Recombinant human Muscle Phosphofructokinase/PFKM/PFK-1(1-780aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11)BSA Free [NBP2-22615]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11)BSA Free [NBP2-22615]

Western Blot: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) [NBP2-22615] - The HepG2 cell lysate (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PFKM antibody (1:500 ~ 1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free [NBP2-22615]

Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free [NBP2-22615]

Flow Cytometry: Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) [NBP2-22615] - Flow cytometry analysis of PFKM in Hep3B cell line, stainingat 2-5ug for 1x106cells (red line). The secondary antibodyused goat anti-mouse IgG Alexa fluor 488 conjugate. Isotypecontrol antibody was mouse IgG (black line).

Applications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Muscle Phosphofructokinase/PFKM

PFKM(phosphofructokinase, muscle) is a regulatory glycolytic enzyme that convert fructose 6-phosphate and ATP into fructose 1,6-bisphosphate (through PFK-1), fructose 2,6-bisphosphate (through PFK-2) and ADP. Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease.

Long Name

Phosphofructokinase, Muscle

Alternate Names

GSD7, PFK-1, PFK-A, PFKM, PFKX, Phosphofructokinase-1, Phosphofructokinase-M, Phosphohexokinase

Gene Symbol

PFKM

UniProt

NP_000280 (Human)

Additional Muscle Phosphofructokinase/PFKM Products

Product Documents for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (2F11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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